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PROMOTIONS FOR THE PURPOSE OF FOUR BY GELPERMEASUREMENT CHROMATOGRAPHY

机译:凝胶色谱法促进四大用途的推广

摘要

Provided herein are elution buffers and methods for purifying viruses using gel permeation chromatography. The methods are useful, for example, in increasing recovery of a virus from a gel permeation chromatography column. The buffers for use in the methods include at least one excipient selected from histidine or sucrose, a divalent cation, a non-ionic detergent, and a phosphate buffered saline.
机译:本文提供了洗脱缓冲液和使用凝胶渗透色谱法纯化病毒的方法。该方法可用于例如增加从凝胶渗透色谱柱中回收病毒。该方法中使用的缓冲液包括至少一种选自组氨酸或蔗糖的赋形剂,二价阳离子,非离子型去污剂和磷酸盐缓冲液。

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