MULTIPLEX REAL-TIME FLUORESCENCE PCR DETECTION PRIMER COMPOSITION AND DETECTION METHOD FOR IDENTIFYING STREPTOCOCCUS SUIS AND SWINE PASTEURELLA MULTOCIDA

摘要

The present invention relates to the technical field of molecular biological bacterial strain detection, and particularly relates to a multiplex real-time fluorescence PCR detection primer 5 composition for identifying Streptococcus suis and Swine pasteurella multocida and a detection method. The present invention can realize precise quantification while providing qualitative detection for Streptococcus suis and Swine pasteurella multocida, and is high in amplification efficiency, high in sensitivity, high in precision rate, good in repeatability, and short in detection cycle. This method can complete detection in 1.5h and can realize real-time monitoring, has 10 very high feasibility and application prospect. It provides a scientific and reliable method for identifying pathogenic microorganism and reducing economic loss of breeding. 17