Method of producing a natural biocomposite from unicellular microalgae of Planktochlorella sp. genus as a modulator of the growth of microorganisms using the phenomenon of metabolic photo-reprogramming

摘要

The object of the invention is the creation of a natural biologically active compound obtained from single-cell microalgae of the genus Planktochlorella sp. as a growth modulator of micro-organisms which may be used in the cosmetic and pharmaceutical industries at the time of hatchinglow-cost photoreprogramming of metabolism. The technology presented in this notification has so far not been applied to the cultivation of micro-algae of the genus Planktochlorella sp. and is based on the use of photomodulation this is the operation of certain light conditions (sourcelight intensity, wavelength, illumination time) to reprogram the metabolism of algae and consequently to obtain the composition with the desired characteristics. The procedure developed for this purpose in accordance with the invention is characterised by this, the cultivation of single-celled microalgae is carried out in full-time light using LED diodes as a source of red light (predominantly 645 nm wavelength) and blue (predominantly 460 nm wavelength) in relation to 3:1 and the total 16000 LUX intensity i.e. 1419 Mmol m-2 s-1, which produces a composition with the following biochemical composition: fat content -10.11 g/100 g biomass,protein content -59.78 g/ 100 g biomass, chlorophyll content and -15.42 mg/l, chlorophyll content b -4.40 mg/l, carotenoid content -1.77 mg/l, saturated fatty acids (SAFA) -77,4%,sum of monounsaturated fatty acids (MUFA) (C18:1 oleic acid) -3,9%,sum of polyunsaturated fatty acids (PUFA) (C18:2 linoleic acid, LA, omega 6) -18.6%. The biochemical profile of the biochemistry plays an important role in reducing the growth of pathogenic bacteria of Enterococcus faecalis, Staphylocoocus aureus, Streptococcus pyogenes, Seratia marcescens, Proteus mirabilis, Bacillus subtilis, Escherichia coli. The use of an ethanol extract from a microalgae formulation of Planktochlorella sp. manufactured according to the described technology allows the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis to be reduced,Staphylocoocus aureus, Streptococcus pyogenes, Seratia marcescens, Proteus mirabilis, Bacillus subtilis, Escherichia coli. Biocomposite obtained according to the described procedure may be used as a precursor. In addition, the technique described in the notification may be used to obtain biomass with a specific fatty acid profile.