Submerged fermentation of rapeseed meal using enzymes and bacteria as a method of reducing oligosaccharides from the raffinose family

摘要

The subject of the application is submerged fermentation of rapeseed meal with the use of enzymes and bacteria as a method to reduce oligosaccharides from the raffinose family in the process of refining rapeseed feed, including post-extraction rapeseed meal with a protein content of not less than 20%. To water with enzymatic additives and a bacterial preparation, each of which is added in an amount from 0.01 to 5% by weight, in particular exogenous 6-phytase with a maximum activity of 1.5 million FYT / kg together with a bacterial preparation including Lactobacillus Brevis , L. Plantarum, L. Kefiri 5.0 x 1010 cfu / g and an enzyme mixture consisting of (endo-1,4 beta glucanase 80,000 U / kg, endo 1,3 (4) beta glucanase, 70,000 U / kg, endo- 1,4 beta xylanase 270,000 U / kg), rapeseed meal is added in the ratio 1: 2 (rapeseed meal / water), and after at least 24 h, the microorganisms are neutralized by heating the obtained mixture at a temperature of not less than 70 ° C for for at least 15 minutes, and preferably the obtained product is dried at a temperature of 45 to 60 ° C until at least 86% dry weight is obtained. The composition of the obtained product is characterized by reduced contents of oligosaccharides from the raffinose family.