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Absorption spectrum scanning flow cytometry

机译:吸收光谱扫描流式细胞仪

摘要

A flow-type particle analyzer for measuring the excitation spectrum of fluorescent particles in a fluid stream, comprising: a) an excitation light source (10) emitting a collimated broad spectrum excitation light beam, b ) excitation optical elements (12, 14) that focus and direct the excitation light beam over a detection region in said fluid stream, wherein said excitation optical elements (12, 14) include a scattering element (12 ) that scatters the excitation light beam spectrum, and wherein said scattering element (12) is oriented so that the resulting spectrum of continuously changing wavelengths is scattered over a detection region of said fluid stream, so that a particle (1) flowing through said detection region will traverse the scattered spectrum of the excitation light beam through the changing wavelengths; and c) optical detection elements (16, 18, 20) that measure the light emitted from the particles (1) in the detection region.
机译:一种用于测量流体中荧光颗粒的激发光谱的流式颗粒分析仪,包括:a)发射准直的广谱激发光束的激发光源(10),b)激发光学元件(12、14),将激发光束聚焦并引导到所述流体流中的检测区域上,其中所述激发光学元件(12、14)包括散射元件(12),所述散射元件散射激发光束光谱,并且其中所述散射元件(12)是定向,使得所产生的连续变化波长的光谱分散在所述流体流的检测区域上,从而流过所述检测区域的粒子(1)将穿过变化波长的激发光束的散射光谱; c)光学检测元件(16、18、20),其测量从检测区域中的粒子(1)发出的光。

著录项

  • 公开/公告号ES2767133T3

    专利类型

  • 公开/公告日2020-06-16

    原文格式PDF

  • 申请/专利权人 BECTON DICKINSON AND COMPANY;

    申请/专利号ES12803171T

  • 发明设计人 NORTON PIERCE;CHEN YONG;

    申请日2012-05-31

  • 分类号G01N21/64;G01N15;G01N15/10;G01N15/14;

  • 国家 ES

  • 入库时间 2022-08-21 11:15:19

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