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SYSTEM BASED ON GFP REASSEMBLY FOR THE STUDY OF TRANS-TRANSLATION ACTIVITY AND THE IDENTIFICATION OF NEW ANTIBIOTICS
SYSTEM BASED ON GFP REASSEMBLY FOR THE STUDY OF TRANS-TRANSLATION ACTIVITY AND THE IDENTIFICATION OF NEW ANTIBIOTICS
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机译:基于GFP的重组系统研究翻译活性及新抗生素的鉴定
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摘要
The present invention relates to a reporter system for trans-translation, based on GFP reassembly and the ability of tmRNA to add a peptide label to a protein which is blocked on a ribosome. The invention also relates to the in vivo or in vitro use of this reporter system for studying trans-translation activity and identifying compounds which are capable of inhibiting trans-translation and therefore likely to be of interest as antibiotics.
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