FLUORESCENCE PCR DETECTION KIT FOR IDENTIFYING DENDROBIUM OFFICINALE KIMURA ET MIGO AND USE

摘要

Involved are a fluorescence PCR detection kit for identifying Dendrobium officinale Kimura et Migo and the use thereof. The kit comprises specific primers and a specific probe for Dendrobium officinale Kimura et Migo, and the nucleotide sequences thereof are as shown in SEQ ID NOs. 1-3. The nucleotide sequences of the dendrobium universal primers and universal probe are as shown in SEQ ID NOs. 4-6. Carrying out PCR amplification by using the fluorescence PCR detection kit can identify Dendrobium officinale Kimura et Migo and other dendrobium plants. The kit pairs the specific fluorescence probe and the template, has a high specificity and selectivity, and has a high amplification efficiency, sensitivity and accuracy, good reproducibility, and short detection cycle. The detection can be completed within 1.5 hours. The kit can detect the DNA amplification reaction in real time, and has very high feasibility and application prospects, ensuring the place of real Dendrobium officinale Kimura et Migo in the market of traditional Chinese medicine.