RECOMBINANT SPIDER SILK PROTEINS, PREPARATION METHOD THEREFOR AND INDUSTRIAL APPLICATION THEREOF

摘要

Disclosed by the present invention are a recombinant spider silk protein series, coding genes thereof, expression and purification methods therefor, and an application of the recombinant spider silk protein series in biological materials such as sutures, bioremediation films and hemostatic materials. The recombinant spider silk protein series described in the present invention is composed of an N-terminal non-repeat region (N-NRT), a core repeat region (REP), and a C-terminal non-repeat region (C-NRT), wherein the core repeat region is composed of 1-30 RepAs and/or 1-15 RepBs, a single RepA amino acid sequence as shown in SEQ ID NO: 1, and a single RepB amino acid sequence as shown in SEQ ID NO: 2. The series of novel spider silk proteins for prokaryotic system soluble expression as designed by the present invention has high fermentation yield and high protein purity, spider silk membranes and spider silk fibers prepared from the spider silk protein series by means of a simple static spinning or wet spinning process have a mechanical strength of 2.5-6 MPa and 40-280 MPa respectively, and exhibit good cell compatibility and hemostatic properties, thus having prospects for industrial application in the medical field.