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METHOD AND KIT FOR DETECTING CHRONIC RHINOSINUSITIS WITH NASAL POLYPS SUBTYPE AND USE OF ALOX15 GENE AS BIOMARKER
METHOD AND KIT FOR DETECTING CHRONIC RHINOSINUSITIS WITH NASAL POLYPS SUBTYPE AND USE OF ALOX15 GENE AS BIOMARKER
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机译:鼻息肉亚型检测慢性鼻窦炎的方法和试剂盒以及使用ALOX15基因作为生物标记物的方法
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摘要
Disclosed are a kit for detecting chronic rhinosinusitis with nasal polyps subtype and the use of an ALOX15 gene as a biomarker, and the method for and the use of detecting the expression amount of the ALOX15 gene in cast-off cells in a nasal cavity. The kit comprises specific primers of the ALOX15 gene. The ALOX15 gene, as a biomarker, can be made into a product for detecting chronic rhinosinusitis with nasal polyps subtype. The detection method of the present disclosure comprises the following steps: extracting RNAs from cast-off cells in the nasal cavity, reverse transcribing the total RNA into cDNA, carrying out real-time fluorescence quantitative PCR amplification of the ALOX15 gene and a reference gene of the cDNA respectively with the specific primers of the ALOX15 gene and specific primers of the reference gene by using a quantitative polymerase chain reaction, and calculating the expression amount of the ALOX15 gene based on a detection result of an amplification product.
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