TEST KIT AND METHOD FOR DETECTING CYP3A4 AND CYP3A5 POLYMORPHIC SITES

摘要

A test kit and method for detecting CYP3A4 and CYP3A5 polymorphic sites, the test kit including CYP3A4*1G specific upstream and downstream primer sequences as shown in sequences 1-4 in a sequence table, CYP3A4*1G specific TaqMan-MGB dual fluorescent probe sequences as shown in sequences 5-8, CYP3A5*3 specific upstream and downstream primer sequences as shown in sequences 9-12, and CYP3A5*3 specific TaqMan-MGB dual fluorescent probe sequences as shown in sequences 13-16. The test kit detects CYP3A4 and CYP3A5 gene polymorphic sites, so as to determine a metabolic capacity of a test object for a fentanyl drug, and thereby accurately and effectively guide medication of the fentanyl drug. The test kit has actual clinical application value. Once applied to clinical diagnosis, compared with the prior art, the test kit has a high matching degree, a low price, pollution prevention, high repeatability, strong specificity, high sensitivity, a rapid detection speed, simple result analysis and high accuracy.