SINGLE-STRANDED DNA APTAMER SPECIFICALLY RECOGNIZING TOBRAMYCIN AND APPLICATION THEREOF

摘要

Provided are a single-stranded DNA aptamer specifically recognizing tobramycin and an application thereof, which relate to the field of biotechnology. On the basis of ap32-34nt, a tobramycin aptamer ap32-15 nt having a greatly shortened sequence length and improved affinity is obtained by means of a manner of removing some of the terminal base pairs and unpaired bases and shortening the length of a secondary structure stem region, and the aptamer ap32-15nt is used in a plurality of tobramycin detection methods as well as the construction of detection reagents. The aptamer has the advantages of high affinity, high specificity, a stable structure, and so on, and the established detection methods have the advantages of a short detection period, high sensitivity, low costs, strong specificity, and so on.