METHOD FOR IN VITRO SCREENING IN-VIVO ANTIBACTERIAL ACTIVITY PHAGES

摘要

#$%^&*AU2020100857A420200702.pdf#####Austracy The present invention discloses a method for in vitro screening in-vivo antibacterial activity phages. The method includes the following steps: SI, selecting an appropriate culture dish, and sterilizing the culture dish; S2, adding a culture medium into the culture dish, wherein the culture medium includes 10-15 g of beef soup, 5-10 g of peptone, 30-50 g of sodium chloride, 10-20 g of glucose and 5-18 g of blood, and mixing the substances to prepare fluid, semisolid and solid culture media; S3, obtaining serum from experiment subjects, dividing the serum into four parts, without performing any treatment on the serum A and B, only inactivating the serum C, and adding a sterilizing agent into the serum D; S4, collecting secretions of infected persons discharged in vitro from the body, wherein the secretions specifically include blood, urine, feces, pus or other secretions; S5, selecting colonies in the secretions, and selecting host bacteria with phages; and S6, respectively performing following treatments on the four parts of serum: still not performing any treatment on the serum A, and adding the host bacteria with the phages into the serum B, the serum C and the serum D. The present invention is high in screening accuracy. 1