A novel LAMP method, primer set and kit for SNP detection

摘要

#$%^&*AU2020101818A420200924.pdf#####ABSTRACT The invention discloses a novel LAMP method capable of detecting SNP, comprising the following steps: (1) extracting DNA as a template to be detected; (2) designing a loop primer probe modified by a a fluorescent group and a fluorescence quenching group, a pair of inner primers and a pair of outer primers the loop primer probe has an RNA base bound to a DNA mutation site; (3)Establishing a reaction system, adding a template to be detected into the reaction system, and detecting whether reactants generate fluorescent signals, wherein if fluorescent signals are generated, target DNA exists in the template to be detected, and if fluorescent signals are not generated, no target DNA exists in the template to be detected.Accordingly, the present invention also discloses a primer set and a reagent kit for detecting Salmonella Pullorum based on the above method. The detection method of the present invention has the advantage of high specificity and high sensitivity.