PCR-RFLP IDENTIFICATION METHOD FOR DIFFERENT SUBGROUPS OF SUGARCANE WHITE LEAF DISEASE PHYTOPLASMA

摘要

Disclosed is a PCR-RFLP identification method for different subgroups of sugarcane white leaf disease phytoplasma, wherein a tuf gene of a sugarcane white leaf disease phytoplasma is taken as an object for designing specific primers, a tuf gene fragment is obtained by PCR amplification, and a restriction endonuclease Msp I is then used to digest the tuf gene fragment obtained by amplification, and different subgroups of the sugarcane white leaf disease phytoplasma are distinguished by a differentiated RFLP restriction map. Therefore, a sugarcane white leaf disease phytoplasma subgroup 16SrXI-B and a sugarcane white leaf disease phytoplasma subgroup 16SrXI-D can be identified without sequencing.