Lipid-based antigen and T-cell receptor on NK cells (LIPID-BASE ANTIGENS AND T-CELL RECEPTORS ON NK CELLS)

摘要

Compositions, methods and uses of genetically modified NK cells for eliciting an immune response against microbially infected cells are provided. In some embodiments, NK cells are recombinant comprising a segment encoding an extracellular single-chain variant fragment that specifically binds to a CD1-lipid antigen complex and another segment encoding an intracellular activation domain and a linker between these segments. It can be genetically modified with nucleic acids. In other embodiments, the NK cells can be genetically modified with a recombinant nucleic acid comprising a segment encoding an α chain T cell receptor and a β chain T cell receptor, and another segment encoding at least a portion of CD3δ and at least a portion of CD3γ. have. Genetically modified NK cells can be administered to a patient infected with the microorganism to trigger an immune response specific to the cell infected with the microorganism.