3 Manufacturing method of 3D lung organoids containing alveolar macrophage-like cells derived from human stem cells

摘要

The present invention relates to a method for producing a three-dimensional lung organoid comprising alveolar macrophages derived from human stem cells. Specifically, the present invention differentiates cells that do not express CRCX4, which is a marker of complete endoderm cells, and the lung organoids prepared according to the method of the present invention include type 1 and type 2 alveolar epithelial cells at the same time. Since it contains alveolar macrophages, unlike lung lung organoids that do not contain existing immune cells, it can be useful for research on the mechanism of related lung diseases, discovering biomarkers, and developing therapeutic agents by implementing an infection or inflammatory response.