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IsPETase IsPETase Method for crystallization of IsPETase protein and IsPETase variants

机译：IsPETase IsPETase结晶IsPETase蛋白和IsPETase变体的方法

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摘要

The invention Is PETase protein crystal production method, a method of screening method for screening Is PETase protein activity modulators using a three-dimensional structure of the protein and Is PETase variants, and PETase activity is increased Is PETase variants and PET decomposition using the same of It's about how. In the present invention, a method capable of effectively producing protein crystals of Is PETase was investigated, and crystals prepared therefrom were obtained. In addition, by identifying the tertiary structure of Is PETase from the crystals, a variant with increased PETase activity was prepared based on this structure. The Is PETase variant can be effectively used in the field of PET degradation.

机译：本发明的 Is PETase蛋白晶体的生产方法，一种利用蛋白质和 Is <的三维结构筛选 Is PETase蛋白活性调节剂的筛选方法。 / I> PETase变体，并且使用相同的方法提高PETase活性 Is PETase变体和PET分解。在本发明中，研究了一种能够有效产生 Is PETase的蛋白质晶体的方法，并获得了由此制备的晶体。另外，通过从晶体鉴定 Is PETase的三级结构，基于该结构制备了具有增加的PETase活性的变体。 Is PETase变体可以有效地用于PET降解领域。 展开▼

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公开/公告号KR102088340B1

专利类型

公开/公告日2020-03-12

原文格式PDF

申请/专利权人 경북대학교 산학협력단;
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申请/专利号KR20190017256

发明设计人 김경진;
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申请日2019-02-14

分类号C12N9/18;C12N15/52;C12N15/70;C12N15/77;C12Q1/44;

国家 KR

入库时间 2022-08-21 11:05:09

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3. IsPETase IsPETase Method for crystallization of IsPETase protein and IsPETase variants [P] . 韩国专利： KR20200017321A . 2020-02-18

机译： IsPETase IsPETase结晶IsPETase蛋白和IsPETase变体的方法

4. METHOD FOR PREPARING CRYSTALS OF IsPETase PROTEIN AND IsPETase VARIANTS [P] . 美国专利： US2020048621A1 . 2020-02-13

机译：异构酶和异构酶变体的制备方法

5. PROTEIN BINDING SPECIFICALLY WITH OSTEOCLASTOGENESIS-INHIBITING FACTOR (OCIF) (VARIANTS), DNA ENCODING ITS (VARIANTS), DNA AS PROBE (VARIANTS), METHOD FOR PREPARING PROTEIN (VARIANTS), METHOD FOR SCREENING SUBSTANCE (VARIANTS), ANTIBODY (VARIANTS), METHOD FOR PREPARING POLYCLONAL ANTIBODY, HYBRIDOMA (VARIANTS), METHOD FOR PREPARING MONOCLONAL ANTIBODY, METHOD FOR ASSAY OF OCIF-BINDING PROTEIN, PHARMACEUTICAL COMPOSITION (VARIANTS) AND MEDICINAL AGENT (VARIANTS) [P] . 俄罗斯专利： RU2238949C2 . 2004-10-27

机译：特定地与抑制破骨细胞增生因子（OCIF）结合的蛋白质，编码DNA的蛋白质（变异体），作为探针的DNA（变异体），制备蛋白质的方式（变异体），筛选物质（变异体），抗体（变异体）多克隆抗体的制备方法，杂交（变种），单克隆抗体的制备方法，卵磷脂结合蛋白的测定方法，药物成分（变体）和药物制剂（变体）

1. Enhanced Extracellular Production of IsPETase in Escherichia coli via Engineering of the pelB Signal Peptide [J] . Lixia Shi, Haifeng Liu, Songfeng Gao, Journal of Agricultural and Food Chemistry . 2021,第7期

2. Toward rational protein crystallization: A Web server for the design of crystallizable protein variants. [J] . Goldschmidt L, Cooper DR, Derewenda ZS, Protein Science: A Publication of the Protein Society . 2007,第8期

3. Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. [J] . Faham S, Bowie JU Journal of Molecular Biology . 2002,第1期

4. Continuous Crystallization of Proteins in a Tubular Plug-flow Crystallizer [C] . P. Neugebauer, J.G. Khinast AIChE Meeting . 2015

5. Development of novel methods and their utilization in the analysis of the effect of the n-terminus of human protein arginine methyltransferase 1 variant 1 on enzymatic activity, protein-protein interactions, and substrate specificity [D] . Suh-Lailam, Brenda Bienka 2011

6. Toward rational protein crystallization: A Web server for the design of crystallizable protein variants [O] . Lukasz Goldschmidt, David R. Cooper, Zygmunt S. Derewenda, 2007

7. Enhanced Extracellular Production of IsPETase in Escherichia coli via Engineering of the pelB Signal Peptide [O] . -1

1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

2. Correlation of Combined Characters of Amino Acid and Whole Protein with Success Rate of Crystallization of iLactobacillus/iProteins [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第4期

3. Prediction of Crystallization Propensity of Proteins from iBacillus haloduran/iUsing Various Amino Acid and Protein Features [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第12期

4. Correlating Combined Features of Amino Acid and Protein with Crystallization Propensity of Proteins from iMycobacterium tuberculosis/i [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第9期

5. Impaired suppressive activities of human MUTYH variant proteins against oxidative mutagenesis [J] . Kazuya Shinmura ,Masanori Goto ,Hong Tao . 世界胃肠病学杂志：英文版 . 2012,第47期

6. 蛋白质突变体库的高通量筛选方法 [C] . WAN Yu-jun ,万玉军 ,WANG Gang . “科技创新驱动发展食品产业—从研究到应用”研讨会暨2016年四川省食品科学技术学会学术年会 . 2016

7. 用交联的方法研究亲免蛋白的相互作用蛋白及mFKBP23蛋白结晶条件的探索 [A] . 顾超 . 2012