A protein secretory factor with a high secretory efficiency and a expression vector comprising the same

摘要

The present invention provides a protein secretion factor derived from the human LBFL313 gene, a nucleic acid sequence encoding the protein secretion factor; And a gene encoding a target protein; and an expression cassette operably linked thereto, to a vector for target protein secretion expression and a transformant cell into which the vector for target protein secretion expression is introduced. In addition, the present invention relates to a method for producing a target protein using transformant cells containing the expression vector. When the protein secretion factor derived from the human LBFL313 gene of the present invention is used, the secretion of the target protein is significantly increased compared to the existing protein secretion factors, and in particular, the antibody uses the protein secretion factor derived from the human LBFL313 gene of the present invention. It was confirmed that the protein secretion factor combination has a superior secretion effect than the secretion sequence of. Therefore, the protein secretion factor of the present invention can be widely used in the field of mass production of proteins, particularly in the field of antibody production.