The present invention relates to a pharmaceutical composition for preventing or treating cancer disease comprising miRNA-550a-3-5p as an active ingredient, a pharmaceutical composition for enhancing anti-cancer agent sensitivity, a method for enhancing anti-cancer drug sensitivity using cancer cells, and a method for screening for anti-cancer drug sensitivity enhancer, According to the present invention, cancer diseases can be treated by regulating the expression of miRNA-550a-3-5p, and in particular, when miRNA-550a-3-5p and anticancer agents are treated together with cancer resistant to anticancer agents such as BRAF inhibitors , Since the anti-cancer agent resistance can be reduced and the sensitivity can be improved, it can be used as a composition for treating cancer diseases or a composition for enhancing anti-cancer agent sensitivity.
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