The present invention relates to a chicken leukemia virus (ALV)-resistant algae produced using the CRISPR/Cas9 system and a method for manufacturing the same. More specifically, resistance to ALV subgroup J (ALV-J) prepared by specifically modifying one or more sites selected from Trp38, Thr37 and Gln40 in the chNHE1 gene of a chicken by gene editing through the CRISPR/Cas9 system ALV-J resistant algae having and relates to a method for manufacturing the same. Avian Leukosis Virus (ALV) resistant birds according to the present invention have a high value as a livestock product because there is no risk of viral infection. In addition, the gene editing method of the present invention can be used for the discovery of a specific genotype capable of inhibiting the binding between the virus and the receptor and the virus-receptor binding study using a cell line, and further producing algae resistant to chicken leukemia virus. It is expected that a system can be established.
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