T T T T A recombinant vector for integration of T cell receptor into the targeted site in T cells and composition for integration of T cell receptor into the targeted site in T cells using the same

摘要

The present invention relates to a recombinant vector for insertion of a T cell receptor (TCR) into a target site in a T cell, and a composition for inserting a T cell receptor into a target site in a T cell using the same, and specifically, four Genetic scissors (CRISPR-Cas9) system to create a recombinant vector containing a homologous recombination template that combines constructs and efficiently insert a TCR pair at a target location in a cell using this It is about. According to the present invention, a construct minimizing a variable region can be produced to reduce costs in the manufacturing and acquisition steps of a variable region, and can be manufactured with high efficiency, and by applying an existing method called Gibson assembly In contrast, it is possible to create a construct that can insert a TCR pair at a target location at a very high speed (single-reaction).