METHOD FOR IDENTIFYING FUNCTIONAL M1 AND M2 PHENOTYPE OF HUMAN MACROPHAGES GENERATED IN VITRO FROM BLOOD MONOCYTES

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, specifically to a method for identifying functional M1 and M2 phenotype of human macrophages generated in vitro from blood monocytes. Proposed method consists in evaluating the effect of cells produced by culturing monocytes in the presence of M1- or M2-polarizing stimuli on a proliferative response of T-lymphocytes in a mixed culture of leukocytes.;EFFECT: method enables to identify M1 and M2 human macrophages with high accuracy.;4 cl, 6 dwg, 3 ex