TEST SYSTEM FOR IDENTIFICATION OF COMMON HEDGEHOG (ERINACEUS EUROPAEUS) TISSUE DNA IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS

摘要

FIELD: biotechnology.;SUBSTANCE: invention represents a test system for identification of common hedgehog (Erinaceus europaeus) tissue DNA in dry fodder and semi-finished meat products, including a polymerase chain reaction buffer, a mixture for its carrying out, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes of region-specific animal genome and for internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×10³ phagic particles per 1 mcl, a negative control sample which is a mixture of recombinant plasmid DNA, containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome with a nucleotide sequence: T4F: 5`-TACATATAAATCACGCAAAGC-3` – forward primer; T4R: 5`- TAGTATGGCTAATCTTATTGG-3` – reverse primer; T4P: HEX-5`-ACATTGGCACTGACCGAGTTC-3`-BHQ1 – probe, taken in volume ratio of 1:1, according to invention for internal control sample phagolysate of bacteriophage T4 is used, and for positive control sample fragments of genomes of native bacteriophage T4 and hedgehog (Erinaceus europaeus) with following nucleotide sequence are used: Hed-F: 5`-AGTCTATTGATTCGAATAGAGC-3` – forward primer; Hed-R: 5`-CATGAGAGGTACTAACCAGT-3` – reverse primer; Hed-P: FAM-5`-CAGGAGCTTTATTAGGTGATGATCAG-3-BHQ1 – probe.;EFFECT: invention allows to expand functional capabilities, increase accuracy of species identity identification, simplification of sample preparation process.;1 cl, 5 tbl