METHOD FOR PRENATAL AND POSTNATAL GENETIC DIAGNOSIS OF NUMERICAL CHROMOSOME ABNORMALITIES IN HUMAN EMBRYOS AND DNA-MICROCHIP BASED ON FULL CHROMIC DNA PROBES

摘要

FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, particularly to methods for prenatal and postnatal genetic diagnosis of numerical chromosome abnormalities in human embryos and DNA microarrays for their implementation. In DNA-microchip substrate is made of glass coated with polylysine, and additionally in probes of slots are probes of positive and negative controls, wherein DNA probes are presented in at least two replications and grouped into a separate rectangular zone, wherein the second DNA probe repeatability is reversed by 180 degrees with a copy of the first replication with respect to the vertical axis, also at the corners of the rectangle there is a positive, presented human genome DNA, and negative, presented DNA phage λ, yeast genomic DNA, control, as fluorescent dyes for labelling can be used Cy3, Cy5, Sulfo-Cy3, Sulfo-Cy5.5.;EFFECT: technical result is simplification of the results obtained as a result of DNA microchip analysis, use of more accessible equipment, reduction of time and financial expenses for creation of DNA-microchip at maintenance of necessary and sufficient accuracy and information value of diagnostics.;4 cl, 2 tbl, 2 ex, 2 dwg