Improved cleaning performance against protein-sensitive soiling V

摘要

The invention relates to proteases comprising an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length and in each case based on the numbering according to SEQ ID NO: 1 (i) at the positions which correspond to positions 9 , 144, 252 and 271 correspond to have amino acid substitutions, preferably selected from amino acid substitutions 9T, 144K, 252T and 271E; and (ii) at at least one of the positions corresponding to positions 18, 38, 48, 89, 101, 131, 145, 147, 149, 162, 166, 172, 189, 192, 205, 211, 215, 217, 218 , 224 and 274 correspond, has at least one further amino acid substitution; as well as their manufacture and use. Such proteases show a very good cleaning performance.