Proteases with improved enzyme stability in detergents and cleaning agents III

摘要

The invention relates to proteases comprising an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length and in each case based on the numbering according to SEQ ID NO: 1 (i) at the positions which correspond to positions 9 , 144, 252 and 271 correspond to have amino acid substitutions, preferably selected from amino acid substitutions 9T, 144K, 252T and 271E; and (ii) has at least one further amino acid substitution in at least one of the positions corresponding to positions 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 and 274; as well as their manufacture and use. Such proteases show very good stability with good cleaning performance.