A microscope comprises a microscope objective, a camera and an optical imaging system for imaging an object through the objective onto the camera along a first optical path. A projection optical system for projecting a test image onto the object through the lens is provided, and the imaging optical system is arranged to project the test image of the object onto the camera through the lens and along at least a portion of the first to map the optical path. A focus adjustment system for focusing the test image on the camera is provided. Using the same objective and the same camera for both imaging and focusing allows a reduction in the costs of the microscope in comparison with known microscopes which provide separate focusing systems.
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