Method for preservation of synovial tissue and separation culture method of stem cell from preserved synovial tissue

摘要

In this study, we propose an effective method for isolation of stem cells such as mesenchymal stem cells.The present invention comprises(1) the process of thinning the synovial tissue collected from the organism so that the maximum diameter becomes less than 1 mm(2) the synovial tissue of the cutFreeze protection solutionThe step of immersing the synovial tissue weight \/ (synovial tissue weight + freezing protective weight) to less than 10% (w \/ W) and 50% (w \/ W);3. Synovial tissue and cryoprotectantContains a process for cryopreservation in environments below 80This is a preservation method of synovial tissue.No selection