首页> 中国专利> 平邑甜茶无融合生殖相关MhSERK4基因和克隆MhSERK4基因cDNA全长序列的方法

平邑甜茶无融合生殖相关MhSERK4基因和克隆MhSERK4基因cDNA全长序列的方法

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摘要

本发明公开了平邑甜茶无融合生殖相关MhSERK4基因和克隆MhSERK4基因cDNA全长序列的方法，苹果属平邑甜茶无融合生殖MhSERK4基因，核酸序列为SEQ ID NO:1。基因的编码区序列为SEQ ID NO:2。基因的编码区序列编码氨基酸为：SEQ ID NO:3。本方法包括：SERK4基因片段的分离；提取平邑甜茶子房的总RNA反转录成cDNA；引物设计及PCR反应；PCR产物的回收和纯化；克隆、测序。在植物的生长发育过程中SERK基因及其家族成员在小孢子发育、体胚发生、激素感应、无融合生殖中都具有一定的功能；对生产上利用无融合生殖固定杂种优势，通过基因工程手段培育优良品种具有重要的实践意义。

著录项

公开/公告号CN104017815B

专利类型发明专利
公开/公告日2016-08-17

原文格式PDF
申请/专利权人沈阳农业大学;
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申请/专利号CN201310641281.4
发明设计人董文轩;张丽杰;王玉霞;高秀岩;刘月学;祁庆钦;王红梅;
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申请日2013-12-02
分类号
代理机构沈阳科威专利代理有限责任公司;
代理人张述学
地址 110866 辽宁省沈阳市沈河区东陵路120号
入库时间 2022-08-23 09:44:39

法律信息

法律状态公告日

法律状态信息

法律状态
2018-01-23

未缴年费专利权终止 IPC(主分类):C12N 15/54 授权公告日:20160817 终止日期:20161202 申请日:20131202

专利权的终止
2016-08-17

授权

授权
2016-08-17

授权

授权
2016-05-18

著录事项变更 IPC(主分类):C12N 15/54 变更前: 变更后: 申请日:20131202

著录事项变更
2016-05-18

著录事项变更 IPC(主分类):C12N 15/54 变更前: 变更后: 申请日:20131202

著录事项变更
2014-10-08

实质审查的生效 IPC(主分类):C12N 15/54 申请日:20131202

实质审查的生效
2014-10-08

实质审查的生效 IPC(主分类):C12N 15/54 申请日:20131202

实质审查的生效
2014-09-03

公开

公开
2014-09-03

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公开

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1. 平邑甜茶无融合生殖相关MhSERK4基因和克隆MhSERK4基因cDNA全长序列的方法 [P] . 中国专利： CN104017815B . 2016.08.17
2. 平邑甜茶无融合生殖MhSERK4基因植物表达载体构建方法与应用 [P] . 中国专利： CN103849635B . 2016.07.06
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
4. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

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