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一种皂荚组培快繁方法

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摘要

本发明公开了一种皂荚组培快繁方法，涉及植物组织培养技术领域。该方法包括以下步骤：(1)取皂荚种子进行消毒处理，之后物理刺破外壳；(2)物理刺破外壳后的种子接种于培养基中，培养成组培苗；所述培养基的配方为：MS+0.5g/LMES+30‑40g/L蔗糖+5‑6g/L琼脂，pH5.8。本发明通过对皂荚种子进行组织培养，建立起皂荚组培快繁体系，使成活率达100％。本发明方法的建立，解决了种子稀少、萌发率低、污染率高、繁殖速度慢的问题，适用于皂荚产业化育苗；本发明的方法使得繁殖速度得到了明显的提高，有利于扩大皂荚的种质资源数量，同时能够降低成本，操作简单，经济效益高。

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公开/公告号CN115413577A

专利类型发明专利
公开/公告日2022-12-02

原文格式PDF
申请/专利权人河北农业大学;
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申请/专利号CN202211052532.0
发明设计人李保会;于丽娜;张洁;徐亚;张芹;郑建伟;
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申请日2022-08-31
分类号A01H4/00;
代理机构北京盛广信合知识产权代理有限公司;
代理人秦全
地址 071000 河北省保定市莲池区河北农业大学
入库时间 2023-06-19 17:48:27

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法律状态
2022-12-02

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KKT is a science wing that tries to solve human problems with a wider perspective aimed at solving problematic human behaviors (Plumb, Stewart, Dahl and Lundgren, 2009). It is seen that there is very little research about the new approach of ACT approach when the aiming country of our country is screened and it is thought that our country will contribute to the field of psychological counseling with the work done. In the scope of the research, experimental and control groups and preliminary test, post-test and follow-up measurements of 2x3 experimental design were used. The study's study group consists of a total of 24 (12 experimental and 12 control groups) university students studying in different departments and levels, continuing their education in the academic year of 2015-2016 in Ağrı province and İbrahim Chechen University in 2015-2016 academic year. The average age of participants in the experiment and control group is 20. 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