Direct electrochemistry of Heme Proteins on Electrodes Modified with Didodecyldimethyl Ammonium Bromide and Carbon Black

摘要

A novel matrix based on commercially available carbon black (CB) N220 and didodecyldimethyl ammonium bromide (DDAB) was shown to be a reliable support for direct electron transfer reactions between screen printed electrode (SPE) and Fe(III)-heme proteins. Cytochrome c(cytc), myoglobin (Mb), horseradish peroxidase (HRP) andudcytochromes P450 (CYP 51A1, CYP 3A4, CYP 2B4) generated well-shaped cyclic voltammograms on SPE/CB/udDDAB electrodes (both in solution and in immobilized state). The attractive performance characteristics of CBudmodified electrodes are advantageous over single-walled carbon nanotubes (SW CNT) based ones. The achieveduddirect electrochemistry of heme proteins on CB/DDAB-modified electrodes provided successful elaboration of theudimmunosensor for cardiac Mb. The immunosensor showed applicability for diagnostics of myocardial infarction displaying significant difference in cardiac Mb content of human blood plasma samples taken from the correspondingudpatients.