Studies on the Components of the Plants on Okinawa which give Poisonous Effect on Fish. ・・・・・・（2）－On the poisonous effect on fish and hemolysis effect of the saponin of Rurihakobe (Anagallis arvensis L.)－

摘要

We extracted saponin from Ruriltakobe (Anagallis arvensis L.) which grows on Okinawa and measured its poisonous effect on fish (Top minnow) and its hemolysis effect. The results are as follows: 1. Hemolysis index is 8895 and foaming stability is 2000. As the poisonous effect on fish (Top minnow), the extracted solution is strong. The solution diluted to 1/1750 brings the fish to death in 70 minutes. 2. Color reaction of saponin and sapogenin. 3. Paper-chromatography of saponin and sapogenin. Solvent: n-butanol: ethanol: water = 1：1：5. Filter paper: Toyo filter paper No. 50, 2×40 cm.Reagent: C_6H_5COCl: H_2SO_4 (d＝1.84): CHCl_8＝3：1：1. Temp.: 18℃. Time: 18 hrs. Ascending. Rf values of saponin: 0.94; 0.84; 0.57. Rf values of sapogenin: 0.50; 0.52; 0.62.　（※表は省略）