Magnetic Resonance imaging (MRI) in detection of _Bifidobacterium longum_ and _Clostridium novyi-NT_ labeled with superparamagnetic iron oxide (SPIO) nanoparticle

摘要

*Purpose:* To investigate the MR imaging of _Bifidobacterium longum_ and _Clostridium novyi-NT_ labeling with superparamagnetic iron oxide (SPIO) nanoparticles. *Materials and methods:* Tubes containing _B. longum_-SPIO, Free-SPIO, _B. longum_ and PYG Medium were incubated under anaerobic condition in _in vitro_ experiment. Transmission electron microscope and Prussian blue staining were used to demonstrate intra-bacteria nanoparticles. R~2~^*^ mapping and R~2~ mapping were reconstructed after MR scanning. _B. longum_-SPIO and _C. novyi_-NT-SPIO were injected respectively _in vivo_ to show whether it might be traced by MR imaging. *Results:* Magnetosomes in bacteria were observed by electron microscopic and stained by Prussian blue staining. At the same concentration of SPIOs, the R~2~^*^ value of _B. longum_-SPIO was significantly higher than that of Free-SPIO (P<0.001), however, the R~2~ value was lower comparing with Free-SPIO (P<0.001). After injection with _B. longum_-SPIO, they could present in tumor and shorten T~2~^*^. *Conclusion:* _B. longum_ and _C. novyi_-NT could be labeled by SPIO and then traced by MRI.