Figure 9: A was acquired at one minute, and B was acquired six hours after the initial fluorescent imaging, the fluorescent signal intensity in the tumor area has no significant difference from other areas of the body expect the liver and spleen. C was acquired 24 h after injection, the fluorescent signal could not be visualized in the mice. D, E and F were acquired from experiments of mice with Huh-7 xenograft tumors blocked using GPC3 antibody presented the same fluorescent imaging characteristics as those in the mice with Huh-7 xenograft tumors.