Isolation of Anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia

摘要

Anthocyanins of Macedonian grape pomace from three varieties “Pinot noir”, “Merlot” and “Vranec” were isolatedudby high speed countercurrent chromatography. After purification of the fractions by means of preparative highudperformance liquid chromatography the structures of isolated pigments were elucidated by electrospray ionizationudmultiple mass spectrometry (ESI-MSn) and nuclear magnetic resonance (NMR) spectroscopy. The major anthocyaninudmalvidin-3-glucoside and the minor pigments delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside,udand malvidin-3-p-coumarоyl-glucoside were isolated.udThe “Color activity concept” was applied and visual detection thresholds of isolated anthocyanins wereuddetermined. The results of the “color activity value” of the isolated pigments and their detection thresholds were inudgood agreement with the color shade of the different varieties of red grape pomace.