Fetal Dexamethasone Exposure Impairs Cellular Development in Neonatal Rat Heartand Kidney: Effects on DNA and Protein in Whole Tissues

摘要

Fetal glucocorticoid exposure causes postnatal growth retardation. To examine themechanisms underlying effects on specific organ systems, the authors administered 0.2 or 0.8 mg/kg of dexamethasone to pregnant rats on gestational days 17, 18, and 19 and assessed three biochemical markers of cell development in heart and kidney of the offspring: DNA content per organ as an index of total cell numbers, DNA per g tissue as an index of cell packing density, and protein/DNA ratio as an index of relative cell size. In both tissues, DNA content became markedly subnormal during the first postnatal week, theontogenetic period of rapid cell division. Partial recovery occurred by the end of the first postnatal month. In the heart, cell packing density was subnormal initially and the cells were significantly enlarged. In contrast, packing density was slightly elevated in the kidney; protein/DNA was increased by the low dose of dexamethasone, but markedly decreased by the high dose. (Copyright (c) 1991 Wiley-Liss, Inc.)