Development of a Novel Technology for the Manipulation of Fish ReproductiveCycles; Controlled Release of Gonadotropin Releasing Hormones

摘要

The document reports on a research study, conducted during the first two years ofan MIT SeaGrant project that was focused on the following areas: (1) Introduction of superactive GnRH analogs (GnRHa) into two polymeric controlled release delivery systems: Ethylene vinyl acetate copolymer (EVAC) and polylactic acid-polyglycolic acid copolymer (PLGA); (2) Characterization of the in-vitro release pattern of the GnRHa from the above delivery systems; (3) Characterization of the in-vivo release pattern of the GnRHa from the above delivery systems in seabream (Sparus aurata) and in Pacific coho salmon (Oncorhynchus kisutch) and of their relationships to gonadotropin secretion; (4) Study of the effect of the above delivery systems on ovulation and spawning in seabream, rainbow trout (Salmo gairdneri), Atlantic salmon (Salmo salar) and Pacific coho salmon.