Improved Method for Screening cDNA Expression Libraries for DNA-Binding Proteins

摘要

The ability to successfully screen a lambda-gt11 complementary DNA expressionlibrary for specific gene products that can bind to selected sequences of DNA depends on radioactive double stranded DNA probes with high specific activity. The authors demonstrate here that probes labeled by the polymerase chain reaction are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated efforts to isolate recombinant phage-containing, putative DNA binding gene products that recognized a 246 base pair transcriptional enhancer region of Rous Sarcoma Virus long terminal repeat.