Activity of gamma-Aminobutyric Acid and Glutamate Transporters in Chick Embryonic Neurons and Rat Synaptosomes.

摘要

The uptake of radioactive gamma-aminobutyric acid (GABA) and D-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on the uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, the authors also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for tritiated GABA and for tritiated D-aspartate with an increasing maximum velocity during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of D-aspartate. (Copyright (c) 1992 Plenum Publishing Corporation.)