Amperometric Biosensors for Detection of Choline and Lactate by Choline Oxidaseand Lactate Oxidase Immobilisation in Microporous Membranes

摘要

This project report deals with the construction of amperometric biosensors forthe detection of choline and lactate, using Choline Oxidase and Lactate Oxidase as the selective element. The enzymes were immobilized in a variety of microporous membrane supports using different immobilization protocols. Microporous membranes for immobilization of biologically active materials are avialable commercially from Millipor (USA) as 'Immobilon' and Pall (UK) as Pall 'Biodyne'. Also 'track-etch' membranes (Cyclopore SA, Belgium) were used in the comparison as a promising new support. The electrochemical sensor response of the membranes was compared with spectrophotometric determination of enzyme activity for the various immobilization methods used. The best membrane for immobilization appeared to be the track-etch membrane, which gave a fast response and a good reproducibility.