Current Status of Test Methods for Detecting Endocrine Disruptors: 'In vitro' Estrogen Receptor Binding Assays. Background Review Document

摘要

The objectives of this BRD are to: (1) provide comprehensive summaries of the published and publicly available unpublished data on the scientific basis and performance of in vitro assays used to test substances for their ability to bind to the estrogen receptor (ER); (2) assess the in vitro ER binding assays considered for their effectiveness in identifying endocrine-active substances; (3) identify and prioritize in vitro ER binding assays that might be considered for incorporation into future testing programs for validation; (4) develop minimum performance criteria by which to judge the effectiveness of proposed in vitro ER binding assays; and (5) generate a list of recommended substances to be used in validation efforts. The data summarized in this BRD are based primarily on information obtained from the peer-reviewed scientific literature. An online literature search was conducted to retrieve records on publications reporting on the testing of substances for their endocrine disrupting effects in vitro.