The Relative Efficacy of Culture Media for the Isolation of Myco-bacterium Tuberculosis from Clini¬cal Material

摘要

The relative efficacy of Lowenstein-Jensen medium, penicillin blood agar, American Trudeau Society medium, Difco Peizer TB medium, Middlebrook 7H9 agar A, and a semi- synthetic autoclavable medium for the isolation of Myco. tuberculosis from specimens submitted to a public health laboratory has been 1 compared. Lowenstein-Jensen was the most sensitive of the media tested though the dif¬ference in positive isolations on this and on the American Trudeau Society medium was hot statistically significant. In our hands, the others were less effective and in some respects more difficult to use. A third slant increased only slightly the proportion of positive find¬ings. The use of two slants with large areas for inoculation appears to be an acceptable technic and this is practicable for laboratories handling large numbers of specimens.