Development and Preparation of ConcentraTed and Purified Vaccines of Adenoviruses (Types 1, 2, 3, 4, 5 and 7) AND Parainfluenza Viruses (Types 1, 2, 3, and 4)

摘要

Stability studies on four experimental type 3 adenovirus vaccines stored at 4C showed good keeping properties for a period of three months. At the end of six months the crude and 20X methanol concentrate showed significant loss in potency, while the 5X methanol and soluble antigen vaccines were unaffected. Similar results were obtained when the four vaccines were lyophilized and stored at 4C. However, in the frozen state (-60C) both methanol concentrates showed a drop in potency after three months, while the crude and soluble antigen vaccines were stable. Attempts to adapt various adenovirus strains to African green monkey kidney met with no success. New attempts are currently being made to passage these strains in African green monkey kidney and diploid cells (WI-38). Master and secondary seeds were prepared for parainfluenza virus types 1, 2 and 3. After having been found free of leucosis, these seeds were used to propagate virus used in a 3X concentrate triple vaccine. The above three types were also concentrated 10X for oncogenicity testing. Attempts are also being made to adapt parainfluenza type 4 (M-25) and respiratory syncytial virus to the embryonated hen's egg. Stability studies on stored parainfluenza 1, 2 and 3 showed no appreciable change for the last six months. Antigen for skin testing was prepared and control testing completed. (Author)