DEVELOPMENT AND PRODUCTION OF MONOSPECIFIC ANTISERA. I. DEMONSTRATION OF DIFFERENT CELL SPECIFICATIONS OF ISO-ANTIBODIES AGAINST LEUCOCYTES. II. DEMONSTRATION OF LEUCOCYTE ISO-ANTIGENS ON SKIN CELLS. III. PRESERVATION OF LYMPHOCYTES WITH MINIMAL LOSS OF VIABILITY. IV. DEVELOPMENT OF THE FIBROBLAST PANEL. V.

摘要

Investigations of the leucocyte antisera were continued. Another 80sera were tested with 20different lymphocyte suspensions; 15of these showed one or more positive reactions. The experimental details obtained so far with 11sera given. Four sera which fulfilled the criteria of 'probable monospecificity',and three others were tested in the 3rd International Workshop on Histocompatibility Testing Torino 1967,the results of which are included. In regard to antiserum preservation, a comparison was made between the cytotoxic antibody activity of serum,citrate plasma, recalcified citrate plasma and recalcified Dowex plasma. Serum and recalcified Dowex plasma proved to be superior. Also given are the results of antiserum storage experiments using different temperatures for different periods. The cell specificity of complement fixing leucocyte antibodies was studied in the immune adherence assay and the cytotoxic antibody test,using pure lymphocytes,pure granulocytes,skin fibroblasts and epidermal cells. Evidence was obtained by absorption experiments,that granulocytes may give falsely negative reactions in the cytotoxic antibody test and that fibroblasts may give falsely negative or abnormally weak reactions in the immune adherence assay. Experiments regarding the preservation of lymphocytes with minimal loss of viability were extended. Optimal conditions for serological purposes were reached,but in more refined techniques slight variations in recovery are still present. Further studies are performed. (Author)