This manual was prepared for Level II mycobacteriology laboratories, those that isolate and identify only Mycobacterium tuberculosis. For ease of reference, the manual is divided into 8 sections: I. Safety, II. Collection and Transportation of Speciments, III. Demonstration and Isolation of Mycobacteria, IV. Specific Identification of M. tuberculosis, V. Drug Susceptibility Tests, VI. Quality Control, VII. Records and Reports, and VIII. Appendix. Each section lists some of the more pertinent references on the subject discussed, methods are presented in sufficient details that reference to another text is only rarely necessary. A number of different test methods, as well as various procedures for performing the same test are presented so that the reader may select the one(s) that are best for his(her) own laboratory situation. Excellent color photographs depict various staining procedures, colony morphologies, and color reactions in the 3 major biochemical tests for M. tuberculosis - niacin, nitrate reduction, and catalase. This manual should be an invaluable aid to the workhorse clinical mycobacteriology laboratory whose job is to isolate, identify and perform drug susceptibility tests on M. tuberculosis.
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