Measurement of the Catalytic (Activity) Concentration of Seven Enzymes in NBS Human Serum SRM 909

摘要

The authors determined the catalytic (activity) concentrations of seven enzymes (ACP, ALP, AlaAT, AspAT, CK, LDH and, gamma-GT) in the NBS lyophilized human serum Standard Reference Material (SRM 909). SRM 909 enzyme activity values should provide the basis for compatibility among methods for determining enzyme values in serum. Separate teams of clinical chemistry experts selected and carried out the analyses for the individual enzymes. The methods used are primarily those recommended by the Standards Committees of the AACC and/or the IFCC, as candidate reference methods; however, a reaction temperature set-point of 29.77 C (the gallium melting point) was used. This monograph describes the serum reconstitution protocol, the specific enzyme methods, and the results of the third (and last) round of the interlaboratory test program, which were used to derive mean values for enzyme catalytic (activity) concentrations in SRM 909.