Continuous, On-Line Interface for Reverse-Phase Microbore High-Performance Liquid Chromatography/Diffuse Reflectance Infrared Fourier Transform Analysis

摘要

An ultrasonic nebulizer with a piezo-electric transducer and a newly designed and constructed continuous on-line collection station have been adapted as an interface between a reverse-phase microbore high-performance liquid chromatograph (RP (mu)HPLC) and a diffuse reflectance infrared Fourier transform spectrometer. The collection station incorporated a directional vacuum port for confinement of the nebulized material onto a small area (3 to 5 mm i.d.) of the diamond powder. The inherent desolvation that occurs upon nebulization of the reverse-phase eluant, along with the vacuum collection system and the diamond powder diffuse reflectance substrate, makes possible the continuous on-line collection and FT-IR analysis of the compounds separated by a RP-(mu)HPLC without any type of initial solute extraction. (Copyright (c) 1986 Society for Applied Spectroscopy.)