Quantitative Evaluation of the Effects of Human Carcinogens and Related Chemicals of Human Foreskin Fibroblasts

摘要

Twelve compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability of human skin fibroblasts in vitro in the presence and absence of human liver S-9 mix. In the absence of the human liver S-9 mix, only seven of the twelve compounds were highly cytotoxic in the 0-100 microgram/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboethoxyamino)-1,4-benzoquinone (AZQ) > cis-platin > N, N-bis(2-chloroethyl)-2-naphthylamine > bis(chloromethyl)-ether (BCME) > acrylonitrile > styrene oxide > aflatoxin B1 (AFB1). The other five compounds, aflatoxin B2 (AFB2), methylmethacrylate, 1-naphthalamine, 2-naphthylamine, and cyclophosphamide, exhibited less than 40% inhibition of colony formation even at 100 microgram/ml of the compound (the maximum concentration of AFB2 used was 50 microgram/ml due to its poor solubility). Cytotoxicity was not significantly altered in the presence of human liver S-9 mix except for AFB1 and styrene oxide treatment. There was a drastic increase in cytotoxicity following treatment with 1 microgram/ml or more AFB1 and a significant reduction in cytotoxicity following treatment with 100 microgram/ml styrene oxide in the presence of S-9 mix. Anchorage independent growth (AI) of treated cells in soft agar was used as a biological end point for the expression of chemical transformation. (Copyright (c) 1990 Princeton Scientific Publishing Co., Inc.)