Gebruik van IRE-Medgenix Serum TSH IRMA voor de Bepaling van TSH in Hielprikbloedvan Pasgeborenen (Use of IRE-Medgenix Serum TSH IRMA for the Measurement of TSH in Bloodspots of Newborn Babies)

摘要

The TSH-IRMA kit, manufactured by IRE-Medgenix is an immunoradiometric assay forthe measurement of thyroid stimulating hormone (TSH) in human serum. The kit was modified and tested for its possible use as routine method in neonatal screening for congenital hypothyroidism (CHT). In the present screening procedure, TSH is measured in a small (1/4-1/8)-inch bloodspot. For this reason bloodspot calibrators of the 2nd IS for TSH 80/558 were prepared on filterpaper. The minimal detectable dose was 0,010 microU TSH in a (1/4-1/8)-inch bloodspot. There was a good correlation between TSH measured in serum and in bloodspots: 'spot' = 1,02 star 'serum' + 0,04 (r= 0,0988, N= 51).