Direct DNA Sequencing of PCR Products

摘要

Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single- and double-stranded PCR products. (Copyright (c) 1991 Wiley-Liss, Inc.)